We use F1/2 for our projects, sounds like your architecture is correct. F1 requires calibration, make sure it is in the code.
In terms of noise, #1 is equal to 0.73mV. In this case, 16 is about 11mV which is acceptable range. 68 is a bit high.
Do the following to see:
- Check the VRef pin to see if you have a glitch. REF3030 is a good part only for low frequency noise. If you have HF noise, it will show up on your VREF (PSRR is <20dB above 10KHz).
- Speed up your ADC sampling rate and compare the data
- Change your ADC sampling time to 3 cycles and repeat the experiment
- Make your software do nothing but this ADC thing and see if you get a better response, other peripherals routinely cause glitches
Come back with these and we can make more suggestions.
Driven shield
It is possible to use shielded wires between the electrodes and the pre-amp without a lot of influence from the shield's added parasitic capacitance (your 2nd dot). The signal itself won't be hurt much because it is very small compared to the common-mode component. To understand this, imagine a tiny differential signal on top of a much, much larger common-mode signal (mostly caused by 50 Hz or 60 Hz mains voltage) and a DC-to-low-frequency component caused by the interaction of the tissue with the electrodes and the body itself. As far as I understand the issue, the interference coupled onto the signal via the cable's capacitance is much worse than having the signal itself fed through the cable capacity.
The trick is to actively drive the cable's shield with the common-mode part of the signal instead of connecting the shield to the pre-amp's ground. Some years ago, I've built such pre-amp with an active guard and was able to use shielded wires as long as 2 m between the electrodes and the first stage of the amp. The schematics can be found in this thesis (not mine, but conveniently includes the most interesting schematics of my EMG amp). Please see fig. 8.7, 8.8 and 8.9 and all the stuff around them in chapter 8. Fig. 8.12 discusses how interference is capacitively coupled onto the signal of interest. Sorry, the thesis is in German, but I hope the images and schematics are international.
A good place to pick up the common mode signal is the "middle" of the gain setting resistor of the initial InAmp (again, see the thesis linked above).
Driven right leg
The right leg is used as a reference to measure signal on left leg, left arm and right arm.
The concept of a driven shield can be extended to actively drive the patient, and the connection is made at the location used as a reference for the signals to be measunred, which is the right leg. This is known as a driven right leg (DRL); there's a good discussion about DRL amps in this article by EDN.
If your measurements are not taken from a human body but from some cells in a dish, you can probably put the DRL electrode onto the bottom or into the jelly / growth medium, close to where your reference electrode sits. This way, you use the same strategy as you would in the sense of a DRL setup.
Notch filter
Also, If the hum is really bad, you can put a notch filter at 50 Hz or 60 Hz into the signal path, but this will also hurt the signal of interest.
Very important safety note: The electrodes must not have any direct galvanic connection to protective earth (PE). This is necessary because once the patient gets connected to a potentially lethal voltage by a fault in another device around the lab, the fault current will have a very good path through the patient and via the electrodes to ground. When talking about a ground reference around the electrodes or the pre-amp, be sure to make this a ground referenced to the pre-amp only and not to the real ground usually known as PE! This usually requires an isolation amp somewhere around or just past the pre-amp, or a digital isolator if you wish to have the ADC close to the pre-amp. More about this in DIN EN 60601-1 and other relevant standards.
Best Answer
This is what the article says: -
The article also says, in relation to devices that produce voltage signals, that: -
Basically it's true but there are some caveats. Consider the noise induced by motors and for this, I reckon induction motors are a likely culprit. They produce magnetic fields that can induce an interfering voltage in a cable whatever the signalling type is.
When voltage signalling is used, the interfering voltage is additive to the signal just like batteries in series are additive. This adds an error.
When current signalling is used AND, providing the induced voltage is not several volts, the current flowing in the cable (due to the signal) remains exactly that current and no voltage interference is seen at the receiving end - this is because of the high-compliance of the 4-20mA current source: -
simulate this circuit – Schematic created using CircuitLab
Hopefully you can see that for a high-compliance current source, interfering voltages that arise in series with the current loop have little effect.
Where does this start to go wrong: -
(1) The compliant current source may need a few volts across it to maintain performance and if the series voltage causes the minimum voltage to drop-below this point there will be glitching introduced onto the signal.
(2) At high frequencies, the compliance will change from theoretically infinite resistance to more like a small value capacitor (due to the transistors and chips in the device). This will allow high frequency interferers to circulate a current through the 100 ohm receiver (R1).
If low frequency signalling is used (with appropriate low-pass filtering at the receive end) HF interference can largely be avoided and it is advised to use screened/shielded twisted pair cable.
High energy E-field interference (as opposed to magnetic interference) tends to be seen as a voltage in parallel with the two wires and this also directly impinges on R1 so shielding and filtering is needed.