SPI bus at 50MHz can easily run a couple of inches thru a few vias without hitch. Wavelength of 50MHz is 6 metres but realistically because fast edges are used you need to think ten times faster. Even so that's a wavelength of 60 cm. Rule of thumb is keep tracks smaller than a half of a quarter wavelength (other folk will use other rules of course) and this means 7.5 cm or 3 inches.
Try and keep clock and data same length and if you hit problems on long stretches you might have to terminate at high frequencies with 10pF in series with 100 ohm (more likely on clock than data).
There is no great theory in my answer, just a whiff of theory and some experiences and he odd rule of thumb. Bottom line - use a ground plane - it's no excuse to say you are not familiar with grounding - get familar - it'll be the difference between something always working and something nearly always never working - it's that big a deal.
In the context of an RF antenna, 'ground plane' refers to any plane-like conductive thing near the antenna, which for, say, FM Radio transmission towers, is actually the ground. Like the kind with dirt that you walk on.
This is because conductive things generally reflect electromagnetic waves. That also means they block them from passing through to the other side. So to be explicitly clear, you may not have any copper at all on any layer or opposite side underneath the antenna. This is the 'copper free zone' in most app notes. The end points of the antenna must also be kept free of any conductive material for some distance. I'd recommend the entire width of the board be free of any conductive elements above the edge of the feeder's ground plane. Which fortunately is probably the case if this is a USB dongle.
Now, in the case of an inverting f antenna, it helps to understand how they actually work.
Current flowing into the actual antenna bit will be mirrored within the ground plane, and this generates a electric field. So the ground plane is really just the other pole of a dipole. The ground plane acts as an asymmetric pole and increases bandwidth, but for the most part, the current flow that matters happens entirely at the edge of the ground plane, parallel to the spine of the F. So, the height (dimension at a right angle to the spine of the F) of the ground plane is unimportant. It's fine if its the ground plane for the entire height of the board (except for the copper free zone around the antenna, of course).
This is good, because it makes our lives a little easier. The only dimension that really matters is the length/width of the ground plane, or parallel to the spine of the F. This is the length of the edge forming our other half of the dipole. And the answer is pretty simple:
As close to λ/4 wide as possible. This is always the distance of the L part of the F. So it will generally be slightly wider than the F's length spine-wise. If it is smaller, you will start losing bandwidth. If it is larger, you will get an increasingly multilobed radiation pattern. For a nice, omnidirectional pattern, quarter-wavelength width is best.
Note, since this is all about those edge currents, you can 'cheat' using some creative slotting of the ground plane along that edge.
Finally, remember that the plane is the other pole of a dipole. So you want it to be free of conductive things obstructing it's 'view' of free space. Ideally, you would make the ground plane be two planes, one on the top layer, and the other on the bottom layer, and stitched together with vias along the edge parallel to the F's spine. This is rarely practical however, so a good compromise is to have a plane on one of the outer-most layers (top or bottom) be the ground plane, and then a second ground plane on an internal layer, as close to the opposite end of the stackup. For a 4 layer board, this would be layers 2 and 4, or alternatively, 1 and 3. This way, on either side, there is as little crap in the way, with one side unobscured and the other with only the component layer. Stitch them together at the edge, of course.
Also, when in doubt, these kind of CAD-able antennas are prime candidates for antenna simulation software. Due to their relative simplicity, you can generally expect simulations to reflect (pun intended) the real performance of the antenna well enough for basic design performance checking. But, PIFAs are generally so simple as to not need even this.
I know this was probably a longer answer than you were looking for, but it's always helpful to understand what is really going on with antennas, as it can prevent overlooking would-be issues and avoiding them. Anyway, I hope this helped!
Best Answer
Thanks for asking an intriguing question. The short answer is "no", you do not need to consider a mirror image of that inverted-F antenna when considering the adequacy of your ground plane.
The inverted-F antenna acts as a short monopole, and as such, you definitely need a ground plane. But without doing a full simulation, you can think of the RF currents induced in the ground plane as a simple set of radial spokes spreading out from the feed point. Your rows of four through-holes would be most troublesome if they were cutting across those radial lines, and least troublesome if they aligned with the radial lines. It could help a tiny bit to nudge them apart, so the ground plane isn't completely interrupted by the row of 4. If your PCB extends at least a quarter-wavelength in all directions, I'd consider it to be quite adequate.