For Git Bash

If you are running msysgit (I am assuming you are) and are looking to run Git Bash (I recommend it over TortoiseGit, but I lean to the CLI more than GUI now), you need to figure out what your home directory is for Git Bash by starting it then type pwd (On Windows 7, it will be something like C:\Users\phsr I think). While you're in Git Bash, you should mkdir .ssh.

After you have the home directory, and a .ssh folder under that, you want to open PuTTYgen and open the key (.ppk file) you have previously created. Once your key is open, you want to select Conversions -> Export OpenSSH key and save it to HOME\.ssh\id_rsa. After you have the key at that location, Git Bash will recognize the key and use it.

Note: Comments indicate that this doesn't work in all cases. You may need to copy the OpenSSH key to Program Files\Git\.ssh\id_rsa (or Program Files (x86)\Git\.ssh\id_rsa).

For TortoiseGit

When using TortoiseGit, you need to set the SSH key via pacey's directions. You need to do that for every repository you are using TortoiseGit with.

As far as I know, private key can only be retrieved at the time you create the keypair (via EC2 web management console or via API commandline).

So you have to save the private key somewhere and be able to retrieve it at work in order to connect to the instance via SSH, since keypairs' public keys are automatically installed on EC2 servers when you launch them.

Hope that helps.